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All TPP-dependent enzymes contain a highly similar TPP-binding domain and its lack in the TKTL1 encoded protein strongly suggests that it is unlikely that TKTL1 is a TPP-dependent protein capable of catalyzing the transketolase reaction

Cause they don't have their health

This is known as general causation

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As you age, collagen production naturally declines by about 1% per year, starting in your mid-20s (NIH)