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In terms of biochemical characteristics, cells undergoing ferroptosis show an imbalance of oxidationreduction levels: a significant increase in intracellular reactive oxygen species (ROS) and a significant decrease in NADPH, and therefore lipid antioxidants can inhibit ferroptosis

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The concentration of Human BMG/b2-MG in the samples is then determined by comparing the OD of the samples to the standard curve

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Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human FE

Elevated levels of reactive oxygen species can activate pro-inflammatory signaling cascades and apoptotic pathways, which in turn exacerbate neuroinflammation, impair neurogenesis, and contribute to synaptic and structural changes implicated in depression (104)