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Successful isolation of monocytes was confirmed with flow cytometry (BD Fortessa) using VivaFix Viability Assay (BioRad), a cocktail of antibodies from BioLegend: anti-CD3 PE (300456), anti-CD14 BV650 (301835), anti-CD19 PerCP-Cy5.5 (302229), and anti-CD56 APC (318309)
Additionally, the assay may produce misleading results when applied to products rich in organic acids, particularly citric acid, due to potential interferences (Corral-Aguayo et al., 2008)
Chan notes
In order to achieve a faster result the right dosage for a certain person must be based on his/her body weight and skin tone
Louis, MO, USA) for 24 h, followed by transfer to PMA-free medium for another 24 h to obtain differentiated human macrophages
Further research is needed to better understand the long-term effects, optimal dosage, and mechanisms of GSH in reducing hangover symptoms and improving alcohol metabolism
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