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The observed differences in free GSH, GSSG, and their respective ratio is likely due to spontaneous oxidation of free GSH in the heparinized plasma rather than a matrix effect of anticoagulant type used during sample collection

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Potential mechanisms for this include 1) decreased glycine availability, to which reduction of AGT expression in steatotic livers could contribute, and which was suggested to have a potential to impair glutathione synthesis in conditions of oxidative stress

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