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Free-amino-acid samples were derived using derivatizing solution (ethanol:distilled H 2 O:triethylamine:phenylisothiocyanate at 7:1:1:1, v/v/v/v) for 15 min

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GHK initiates the cellular downstream pathway and exerts bioactivities through binding with cell surface receptors ( In vitro and in vivo studies proved that this combination stimulates the synthesis of collagen and glycosaminoglycans (Figure 1), respectively