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The standard western blot protocol was performed as our previous study 6

Store reconstituted TB-500 refrigerated and use within the beyond-use date on the pharmacy label

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In occlusion/occlusion-like syndromes affecting both periphery and brain (portal hypertension, caval hypertension, intracranial hypertension), BPC-157 simultaneously normalized blood pressure disturbances, healed gastrointestinal and liver lesions, minimized brain lesions and hemorrhage, prevented thrombosis, and preserved neurological function

Prior to euthanization, the animals from the 30, 90, 190, and 360 day post-injury interval groups were placed in a wooden box that allowed for tail exposure