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The concentrated enzyme was loaded onto a Superdex 200 Prep Grade column equilibrated with 25 mM HEPES, 150 mM NaCl, and 20 mM cysteine at pH 7.4

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Based on the aforementioned literature on ASD pathophysiology, we hypothesized that adults with ASD would show evidence of a dysregulated Gln-Glu cycle and heightened E/I balance, manifested by an increase in Gln levels, accompanied by potential alterations in Glu and lower GABA levels