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The homogenates were centrifuged at 20,400 g for 20 min at 4 C, and the resulting supernatants were appropriately diluted and used in the assays, which were conducted according to the manufacturers instructions

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Many pharmaceutical companies have tried to develop drugs that target Mcl-1, but this has been difficult because the interaction between Mcl-1 and its target protein occurs in a long stretch of 20 to 25 amino acids, which is difficult to block with the small molecules typically used as drugs

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