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Screening of GST fragments which can inhibit the GST activity 150 single positive clones (grown on the RGB plates), that could tightly bind to the glutathione Sepharose 4B, were picked up from the plates, and cultured separately in 50 ml of IMC medium containing 100 g/ml ampicillin for 15 hours at 25C, then induced with 100 g/ml tryptophan for 6 hours

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Whey protein provides cysteine, a critical precursor that promotes glutathione synthesis

Anyway, that's a bit about that gene

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A byproduct of this metabolically intensive process is creation of free radicals