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Cells were lysed in 100 L ice cold 8 M Urea/50 mM Tris HCl lysis buffer (Fisher Scientific) with 1:100 of Halt Cocktail Inhibitor, EDTA-free (100X) (Thermo Scientific)
Symptoms of vitamin B 12 deficiency Vitamin B 12 deficiency results in impairment of the activities of vitamin B 12 -requiring enzymes
Potency decreases over time even under optimal storage
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In six instances, ASVs that had not been assigned a genus by DECIPHER mapped consistently to a genera and species across the BLASTn and BLCA methods