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269 The multilineage differentiation potential of aged HSCs is impaired, showing a bias toward myeloid differentiation (erythroid, granulocytic, and megakaryocytic lineages), whereas the differentiation ability toward lymphoid lineages (T cells, B cells) is significantly reduced

Both single-cell suspensions were spun at 200 g for 5 min, resuspended in DM medium as described above but without A8301, and then manually counted with a haemocytometer to determine cell concentration

aliquot for long-term storage Storage and Handling Proper storage and handling of bacteriostatic water and reconstituted peptides: Bacteriostatic Water (Unopened) Store at room temperature (1530 C) Protect from direct light (store in original box or dark cabinet) Do not freeze (may compromise benzyl alcohol efficacy and vial integrity) Check expiry date before use

May 2022;8(3):1025-1035

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Tanchoco CC, Cruz AJ, Duante CA, Litonjua AD