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The resulting single-stranded cDNAs were used as templates in PCR reactions using gene-specific primers to amplify TGR (forward 5-CTATTTCCGTAGACGTCTGT-3 and reverse 5-AATACAGTTTCCTTCCCGTT-3) or GAPDH (forward 5-GTTTTGGTCGTATCGGGAGA-3 and reverse 5-ATGCGTTAGAAACCACGGAC-3)

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doi: 10.7314/APJCP.2014.15.9.4019