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Mistake 2: Shaking the vial to dissolve Vigorous shaking creates shearing forces between liquid layers that can damage peptide bonds
Consequently, this causes ischemic hypoxia and exacerbates stroke-associated brain edema and neuronal injury (Paulson et al., 2006
Typically, it is taken with water after a meal
Quantitative real-time PCR (qRT-PCR) analysis Total RNA was isolated from cortical samples by using RNAiso plus (Takara, Shiga, Japan) according to the manufacturers instructions
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