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Bioinformatic analyses Human reads were removed by mapping to the hg19 release of the human genome using BBTools (available at Because BoosterShot technology is optimized for fecal samples, taxonomy was re-annotated the reads using Kraken2 (26) with confidence set to 0.5 and Bracken, based on the Human Oral Microbiome Database v9.0.3

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2 Key Facts about Tobacco Use among Children and Teenagers Every day, almost 2,500 children under 18 years of age try their first cigarette, and more than 400 of them will become new, regular daily smokers

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