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Glucose deprivation experiments used glucose-free DMEM (Thermo, 12100-061) containing 10% dialyzed FBS (Gibco, 30067334)

We have validated that this TYR-catalyzed prodrug activation approach could effectively retain the intracellular drug concentration to resensitize the drug-resistant melanoma cells to chemotherapeutics both in vitro and in vivo

[DOI] [PubMed] [Google Scholar] 10.Wisor J.P., Rempe M.J., Schmidt M.A., Moore M.E., Clegern W.C

The effect of each treatment on cell viability was assessed by measuring protease activity using the cell permeant substrate GF-AFC using the ApoTox-Glo Triplex Assay

This cholesterol enrichment leads to cholesterol accumulation in glioblastoma infiltrating macrophages and microglia-derived tumor-associated macrophages (MN-TAMs) (33, 34)

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