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One review concluded that vitamin B12 plays a key role in fat metabolism, noting that a deficiency could be linked to increased fat accumulation and obesity ( Another animal study found that severe vitamin B12 deficiency led to a significant increase in body fat percentage and cholesterol levels in pregnant mice ( Whats more, one animal study showed that administering B vitamins to rats fed a high fat diet reduced weight gain and increased levels of several enzymes that influence metabolism, compared with a control group ( However, current evidence is lacking, and additional high quality studies are needed to determine how vitamin B12 may affect metabolism and body fat in humans