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5 M, Invitrogen) for 20 min at 37 C, washed once with PBS, and resuspended in RPMI 1640 medium supplemented with 10% FBS

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I believe these menthol-flavored products represent one of the most common and pernicious routes by which kids initiate on combustible cigarettes, he said in a statement at the time

Protein Expression and Purification, 155 (March), 5458