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Immunofluorescent staining in iPSC-derived brain organoids Day 50 and day 100 of cBOs of different conditions embedded in OCT were sectioned at 10 m using the cryotome (Microm HM525, Thermo Fisher Scientific)

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46,47,48 In 2005, the discovery of pseudouridine () modification addressed the immunogenicity issue of in vitro-synthesized mRNA, 49 leading to the initiation of the first human trial of an mRNA vaccine against melanoma in 2008