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For batch fermentation, pre-cultured cells were harvested and inoculated into main cultures to an initial optical density at 600 nm (OD 600 ) of 1.0

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These small unmyelinated C-fibres and thinly myelinated A-fibres are the primary fibres affected in diabetic peripheral neuropathy, sarcoidosis-associated neuropathy, and other small fibre neuropathy conditions, making their regeneration a central focus of ARA-290 research

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