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Immunocytochemistry staining of hepatic marker proteins was performed to assess the cell maturity of differentiated HLCs

That matters because connective tissues remodel slowly

doi:10.1016/j.mri.2008.06.002

Since then they have been the subject of great interest and study

Topical applications may show initial improvements in skin texture and hydration within 2-4 weeks, with more significant changes in collagen production and skin firmness becoming apparent after 8-12 weeks of consistent use

While individual response varies, most patients follow a general pattern: Subtle improvement in skin freshness Skin may appear healthier and more hydrated At this stage, changes are often noticeable to the patient before others see them