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Since patients who developed EGFR mutant lung adenocarcinomas with acquired resistance to EGFR inhibitor drugs (gefitinib or erlotinib) exhibit increased copy numbers of MET, c-Met-HGF inhibitors are used for lung cancer treatment alone or in combination with other drugs [148]

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To exclude that the observed metabolic phenotype was cell line specific, we validated the findings in two isogeneic spontaneously metastatic sublines, D2A1-m1 and D2A1-m2, generated from the poorly metastasizing murine-derived D2A1 cell line by serial in vivo passaging (Jungwirth et al, 2018)

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This window is sufficient to observe early changes in body composition and GH axis responsiveness