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"The smoking area is a kind of a communication space for the Japanese people because usually they can't communicate with different types of people," Furuta says, "especially the higher class of the company." It's not exactly a movement yet, but Abe and Kota are taking their quiet bubble crusade to shopping centers, festivals and eateries in hopes of showing this stubbornly tobacco-friendly nation an alternative to one of their preferred social pastimes.

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Slides were then cooled to room temperature on ice and washed three times with TBST (1 TBS, 0.5% Triton X-100) before blocking in Block-B buffer (1 TBS, 3% BSA, and 0.1% Triton X-100) for 30 min at room temperature