Lean proteins: Eggs, chicken, and seafood
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Medically reviewed by Lauren Okafor | MD , The Frank H Netter MD School of Medicine, Loyola University Medical Center on March 15th, 2026
2.4 Western blot analyses Whole cell protein lysates were prepared by lysis in radio-immunoprecipitation assay buffer (150 mM NaCl, 2 % NP-40, 0.5 % sodium deoxycholate, 0.1 % SDS, 50 mM Tris pH 8.0, 1 mM PMSF, 1 g/ml aproptinin, 1 g/ml pepstatin, 1 g/ml leupeptin, 1 mM NaF, 1 mM sodium orthovanadate, 0.1 mM sodium molybdate, 10 mM sodium pyrophosphate) at a density of 4 10 7 cells/ml on ice, followed by addition of equal volumes of Laemmli protein sample buffer (60 mM TrisCl pH 6.8, 2 % SDS, 10 % glycerol, 5 % -mercaptoethanol, 0.01 % bromophenol blue) and heated to 95 C for 5 min prior to separation on SDS-PAGE gels and transfer to 0.45 m nitrocellulose membranes
Additionally, individuals with PCC exhibited significantly elevated levels of six proteinsPCSK9, CST3, C1Q, CPB2, KNG1, and GAPDHassociated with glycolysis, complement and coagulation cascades, and inflammatory pathways
Following this segment, theres a collagen-mimetic domain characterized by 22 recurrent sequences
