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Ice nucleation measurements were conducted immediately after heat treatment to determine the ice nucleation temperature of the denatured proteins and peptide

Alternatively, acetylation sites in the CE-clan protease domain might also interfere with the autoproteolytic mechanism

That removes the most common escape hatch they just didnt use enough and leaves the more uncomfortable possibility that the original effect was smaller than reported, or absent

Acknowledgements We thank Yale School Medicine, Yale West Campus, Systems Biology Institute and Cancer Biology Institute for the instrumentation supports

avenae) to explore the expansion of genes encoding glutathione synthetase-like domains (Pfams PF03917 and PF03199 Fig 1)