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Protein Proteomic Control Set size:4 x 2mg Centrifuge 1–2 ml samples of

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Description

Centrifuge 1–2 ml samples of the overnight culture

Thirsty-Swabs may be squeezed and reused

c Expressing plasmids corresponds to observation of a heterologous protein in the total cell pellet on Coomassie-stained SDS-PAGE following growth of a colony in LB+ampicillin medium and induction with IPTG

Colonies were inoculated into LB-Miller medium and grown at 37°C for induction

(1989) In: Molecular Cloning: A Laboratory Manual (2nd ed

Protein Proteomic Control Set size:4 x 2mg Centrifuge 1–2 ml samples of

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