Nat Commun 2016;7:11398
Validation of pivotal genes RT-qPCR analysis was conducted on chorionic villus tissues obtained from both the missed abortion and control groups
There is no standard dose of arginine
The cells were incubated overnight at 4 C with one of the following primary antibodies: rabbit anti-SMAD2 (Cell Signaling Technology, Danvers, MA, RRID: AB_823638, 1:200 dilution), rabbit anti-SMAD3 (Cell Signaling Technology, RRID: AB_2193182, 1:200 dilution), rabbit anti-phospho-SMAD2 (Abcam, RRID: AB_2732791, 1:100 dilution), or rabbit anti-phospho-SMAD3 (Cell Signaling Technology, RRID: AB_2193207, 1:100 dilution)
Author contributions SA: Methodology, Writing original draft
Self-prescribing B12 injections for weight loss purposes is not supported by clinical evidence and may delay diagnosis of underlying conditions contributing to weight concerns or low energy levels