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The resulting amplicon was digested with Nco I and Hin dIII and inserted into vector pET-28b, generating the pET-28b-MBP-TEV-R9 plasmid for expression of TEV with a His 6 -tag at its N-terminus and nine consecutive arginine residues at its C-terminus

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Chewing tobacco has remained popular among tobacco users because of its strong flavour and the longer-lasting experience it provides

Waste facilities that collect large volumes of vape products can minimize incidents by isolating loose batteries, using thermal monitoring systems, and ensuring staff are trained in battery fire protocols

Filtering procedures were performed separately for both polarity modes and lipidomic assessment, prior to merging of the final lipidomic data set

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