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Mice showed no adverse physical effects in any of the treatment or control arms, and body weights remained stable throughout the experiment (Fig

The pituitary responds as if the body is producing GH naturally, which means the downstream IGF-1 response and tissue effects follow normal biological patterns

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At the same time, IGFBP-3, the binding protein of IGF-1, can regulate the IGF-1 activity in tissues to some extent, and the molar ratio of IGF-1/IGF-BP-3 can unveil the number of IGF-1 with biological activity [30, 31, 32]

Front Psychiatry (2018) 9:559