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DOI: 10.1111/j.1742-4658.2009.07472.x 31

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Due to intrinsic Cpf1 ribonuclease activity that facilitates crRNA processing and an array of donor DNA sequences for homology-directed repair of double-strand breaks generated by Cpf1, the multi-CRISPR/Cpf1 system is more efficient for multiplex deletion of up to four genes ( CRISPR-based genomic editing has been developed for multiplex gene editing in Bacillus ( rib A, B, and H genes ( B

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C., Viegas, C