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These have been suggested to be responsible for increases in the frequency and severity of asthma-related exacerbations due to smoke exposure [33, 34]

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We performed quantitative reverse transcription-polymerase chain reaction (RT-PCR) analysis to determine the expression of the proinflammatory cytokine gene IL6 , proinflammatory chemokine gene IL8 , and Toll-like receptor genes TLR1 , TLR2 , and TLR4

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