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To test this, reverse transcriptase (RT)-PCR products of the region containing the two mutations were digested with Hae II

ABRs were recorded using subdermal needle electrodes inserted at the vertex (active), ipsilateral mastoid (reference), and contralateral mastoid (ground), where the responses were amplified 10,000 , band-pass filtered from 0.3 to 3 kHz, and averaged across 512 repetitions at a rate of 21/s

The absence of safety data in these populations should inform clinical decision-making and prescribing practices

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