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The final, crucial step is labeling

However, observed barrier defects and associated diarrhea even in these mice did not result in changes in claudin-1, -2, -3, and -4 expression and cellular distributions, but rather correlated with the rearrangement of cellular distribution of occludin, and to some extent with JAM proteins

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Performance of the hCMEC/D3 and immortalized human brain endothelial cell lines (HBMEC/ci), measured with TEER and expression of tight junction proteins, was enhanced by compounds, such as hydrocortisone or lithium (Furihata et al., 2015

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The reaction may happen right away or it may start hours after the injection