ROS levels are measured as oxidation index of a red fluorogenic reagent by mitochondrial superoxide of live cells (that gives a red fluorescence)
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Healthy endothelium has anticoagulant properties that prevent the adhesion of leukocytes and platelets to the vessel walls, contributing to resistance to thrombus formation, and regulating inflammation, and vascular smooth muscle cell proliferation
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Dysregulation of calcium signaling pathways can induce ROS production, which is sufficient to cause oxidative stress in aging oocytes or trigger FAS ligand-mediated oocyte apoptosis [38]
It is estimated that 25% of the oxygen that is passed through the electron transport system inside the mitochondria results in superoxide[3]