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Following extraction, mRNA expression levels were quantified using SYBR Green-based quantitative real-time PCR (qRT-PCR) performed on the StepOne system (Applied Biosystems), which is recognized for its precision in gene expression analysis

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We identified the limitations of existing treatments and explored potential future developments for novel SCD therapies

If the predetoxification plasma level is below the broken line described above, there is minimal risk of hepatic toxicity and acetylcysteine treatment can be discontinued

PMCID: PMC4156804