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The column was then washed with buffer A (300 L, 2,000 g , 2 min), and 10 mM aqueous NH 4 HCO 3 containing 5% CH 3 CN (300 L, 2000 g , 2 min), before peptides were eluted from the column as 15 fractions using 300 L buffer containing an increasing concentration of CH 3 CN in 10 mM NH 4 HCO 3 (%CH 3 CN = 7.5, 10, 12.5, 15, 17.5, 20, 22.5, 25, 27.5, 30, 35, 40, 45, 50, 75) (2000 g , 2 min)

doi: 10.3389/fmolb.2021.765970 Received 27 August 2021 Accepted 01 November 2021 Published 22 November 2021 Volume 8 - 2021 Edited by Marc Diederich, Seoul National University, South Korea Reviewed by Dan Herschlag, Stanford University, United States Adrian Drazic, University of Bergen, Norway Updates Copyright 2021 Mannervik, Ismail, Lindstrm, Sjdin and Ing

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