Subsequent chromatin immunoprecipitation assay (ChIP) showed that NRF2 binds to the locus but not the downstream exon 7 region (Fig
Methods TCGA database mining We downloaded the processed TCGA RNA-seq gene expression data for 50 normal and 373 tumor LIHC samples from The Cancer Genome AtlasData Portal ( We log 2 -transformed the normalized read counts (per million reads mapped) for RNA-seq data (all values less than 1 were assigned value 1 before transformation) but carried out no further normalization
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Since the methionine cycle and the TSS pathway are closely connected with the former providing homocysteine for utilization in the latter, we determined the expression of the factors involved in the methionine cycle
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