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90 L of the cell culture was added into each well containing 10 L of the azido-tagged dye at 500 M in 96-well plates, covered with plastic film, and incubated for 1 h at 37 C in a shaker incubator

doi: 10.3389/fcvm.2023.1118625 Received 07 December 2022 Accepted 09 March 2023 Published 21 March 2023 Volume 10 - 2023 Edited and reviewed by Mary G

CentralGoogle Scholar Soderborg TK, Clark SE, Mulligan CE

Its performance characteristics include: Glutathione coupled to Sepharose High Performance, excellent for high resolution purification of GST-tagged proteins Easy one-step purification of GST-tagged proteins and other glutathione S-transferase or glutathionedependent proteins Prepacked GSTrap HP columns for simple operation with a syringe, pump or chromatographic system, such as KTAdesign These features make Glutathione Sepharose High Performance the ideal medium for high resolution purification of GST-tagged proteins, glutathione S-transferases and glutathione binding proteins

Choline is essential for transporting fat out of the liver and into circulation

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