Harvested supernatants were used for gene knockout
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The BBB employs various transport systems, such as carrier-mediated transport (CMT), receptor-mediated transport (RMT), active efflux transport, vesicular trafficking by transcytosis, and ion transport, to facilitate the entry of essential substances into the brain parenchyma and the efflux of metabolic waste and endogenous neurotoxins [19, 23]
J Altern Complement Med 2013 May;19(5):459-463
It is not fine when sterility and preservative concentration actually matter and you have no way to check what is in the vial
Brain tissue (100 mg) was taken in 900 microliters of PBS (1:9) ratio in a glass homogenizer on ice and centrifuged at 2,000 rpm for 5 minutes