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At 15 min post-ligation, venograms were taken, captured, and digitized into files on a personal computer and were analyzed using ISSA image software (Vamstec, Zagreb, Croatia)

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All other results were statistically analyzed using the one-way analysis of variance (ANOVA) (biological replicates n = 3 or 5), followed by Tukey's multiple comparison test using GraphPad Prism v.9

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Peptide therapy uses targeted chains of amino acids to naturally stimulate cellular repair, hormone balance, fat metabolism, and anti-aging effects