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Filamentation is associated with a shared cytosol and is caused by a variety of stressors including DNA damage (the SOS response), mediated by RecA

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cDNA constructs of GST fusion proteins were incorporated in pGEX-6p-2 plasmid (GE Healthcare Life Sciences, Little Chalfont, USA) and transformed a bacterial line BL21

Therefore, balancing Ero1 and GSSG oxidation of PDI could regulate the [GSH]:[GSSG] ratio

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