Materials and methods Cell culture and compound treatment HepG2 cells (ATCC, catalog: HB-8065) were cultured with 90% Eagles Minimum Essential Medium (EMEM, ATCC, catalog: 30-2003) and supplemented with 10% fetal bovine serum (FBS, Cytiva, catalog: SH30070.03) and 100 U/mL penicillin-100 g/mL streptomycin (Thermo Fisher Scientific, catalog: 15140122)
These established short-term safety and pharmacology context but were not designed to demonstrate weight loss efficacy
De Gregorio C, Marini H, Alibrandi A, Di Benedetto A, Bitto A, Adamo EB, et al
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"Glutathione Synthesis and Diet," Robb Wolf: Revolutionary Solutions to a Modern Life, under Silvagno, Vernone, Pescarmona
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