Helps regulate female hormones
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In recent years, the role of GPX3 in oxidative stress has been reported, for example: GPX3 can inhibit the expression of HIF1- by regulating ROS, thereby inhibiting the growth of melanoma cells ( As mentioned earlier, the vast majority of GPX3 is secreted by the renal proximal convoluted tubules, so we speculate that the kidney may be a highly expressed organ of GPX3, and to test our hypothesis, we analyzed the GSE111159 dataset (This dataset mainly detects the gene sequencing of 10 tissues and organs of mouse kidney, liver, brain, lung, thymus, muscle, spleen, stomach, heart and testicle under physiological conditions to understand the gene expression of each tissue and organ) in the GEO database (Gene Expression Omnibus, We found that GPX3 had the highest level of expression in kidney tissue under mouse physiology (Figure 3A), which is consistent with the results of the analysis by Kim et al
30 and 31) were tested
Cell proliferation assay (CCK-8) The medium in a 96-well plate was replaced with 100 L of the complete medium and Cell Counting Kit-8 (CCK-8) reagent mixture (9:1), and the plate was incubated in 5% CO 2 for 24 h at 37C
Fitness 17 GallowayS
