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Glutamate is precursor for glutathione biosynthesis

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most preclinical studies use young adult animals rather than aged subjects with accumulated pathology

Using a tunneled line reduces the risk of infection as compared to other forms of access, as bacteria from the skin surface are not able to travel directly into the vein

On the day of assay, the mannitol and sucrose (MAS) buffer (1) was prepared by diluting sterile-filtered MAS 3 in distilled water, resulting in a solution with the following final concentrations: 220 mM mannitol, 70 mM sucrose, 10 mM KH 2 PO 4 , 5 mM MgCl 2 , 2 mM HEPES, 1 mM EGTA and 0.2% w/v fatty-acid free BSA (3117057001) (pH 7.2), and warmed to 37 C