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[6] Absolute contraindications include hypercalcaemia, hypervitaminosis D, idiopathic infantile hypercalcaemia, or documented hypersensitivity to vitamin D or components of the oil vehicle because additional vitamin D would worsen calcium overload or provoke severe allergic reactions

After TMB substrate solution is added, only those wells that contain Mouse C5a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color

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