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Materials and methods This retrospective study included 47 patients (229 intervertebral discs from L1/2 to L5/S1

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Some of them are used as building stones for Vitamin A (retinal) that also has antioxidant properties, independent of the oxygen concentration [3]

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the membranes were then washed several times with water and TBS (PBS, 0.05% Tween-20), a 1 :5000 dilution of horseradish-peroxidase conjugated goat anti-rabbit antiserum was added, and the membranes further incubated for 1 h at room temperature

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