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The basis set was simulated on an 81 3 grid using the fast 1-D spatial projection method 70 , using the FID-A toolbox and based on sequence timing and radiofrequency (RF) pulse shapes and included: alanine, ascorbic acid, aspartate, creatine, phosphocreatine, GABA, glucose, glutamine, glutamate, glutathione, glycerophosphocholine, myo-inositol, l-lactate, N -acetylaspartate, N -acetylaspartylglutamate, phosphocholine, phosphoethanoamine, scyllo-inositol and taurine with the default macromolecule stimulation parameters from LCModel

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Then, after protein quantification of the lysate, MDA working solution was added and heated at 95 C for 60 min