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1 H NMR (400 MHz, D 2 O) 7.78 (s, 2H), 7.01 (s, 2H), 4.71 4.60 (m, 2H), 4.44 4.03 (m, 3H), 3.80 3.74 (m, 4H), 3.32 2.88 (m, 10H), 1.85 1.11 (m, 18H) 13 .C NMR (151 MHz, D 2 O) 178.88, 172.79, 172.16, 172.10, 167.38, 167.21, 134.76, 134.56, 129.96, 129.66, 117.45, 54.20, 54.17, 53.68, 53.46, 53.08, 40.64, 40.59, 39.33, 39.28, 37.38, 30.79, 30.39, 30.37, 28.05, 27.65, 27.48, 27.40, 22.63, 22.18, 22.04

Quantitative Real-Time PCR (qRT-PCR) was used to measure mRNA expression of SASP factors (IL-1, IL-6, TNF-, TGF-) in TM3 cells, normalizing data to GAPDH

Glutamate is converted to intermediate -ketoglutarate (KG) of the TCA cycle by glutamate dehydrogenase (GDH) or alanine or aspartate aminotransferase (TA)