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4T1 cells were pretreated with free OLE, PEG-T ECM -NS/OLE, and PEG-T ECM -NS/OLE pretreated with pH 6.8 buffer and 100 M H 2 O 2

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Specifically, demethylation of the NRF2 gene promoter region can activate NRF2 expression, enhancing the binding of transcription factors and regulatory proteins to the promoter, thereby increasing the transcription and protein expression of NRF2

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