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If you arent seeing the results you expected, several explanations may exist

Consequently, the proven leishmanicidal effect of IONPs in topical treatments suggests two possible mechanisms: either the free iron resulting from the degradation of the particles reaches the infected areas and act on these macrophages, or the key molecules for parasite control, such as ROS and NO, are generated in more superficial layers and diffuse towards the areas where the parasite is located

A classical drug is like a key in a lock, it has one target receptor and binds there

While this purification is conceptually very similar to many other types of affinity purifications, there are a few specific things to keep in mind when purifying GST-fusion proteins

10.4414/smw.2014.14021 34 LevineR

Then apply a plain moisturizer on top