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Wybr midzy nimi zaley od projektowanego ukadu eksperymentalnego czy badacz potrzebuje jednego szlaku, czy obu jednoczenie

To ensure that the primers produced a single and specific PCR amplification product, a melting curve was performed at the end of the PCR cycle

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For MS/MS, the following settings are used: isolation window width 1.2 m/z with an offset of 0.5 m/z, stepped NCE at 10, 15, and 25 a.u., minimum AGC 5E 2 , and dynamic exclusion of previously sampled peaks for 8 s